FAQ

service

  Tel:18962587269
  Email:contact@readcrystal.com
  AddressChangshu High-tech Industrial Development district, Suzhou, Jiangsu Province 1001, Building 6, No.88 Xian Shi Road

FAQ
Services
1. How can I order your services?
A: You can call our official contact number +8618962587269 to consult. You can also leave a message online, we will contact you by phone/email as soon as we receive the message.
2. What if I have special or additional needs?
A: Our aim is to provide customized one-stop R&D services for our customers. If you have any special or additional needs, you can inform us and we will try our best to help you solve the problem.
3. How do I send my samples to your company for testing?
    A: You need to mail to our company. Depending on the nature of your samples and your own needs, you can choose either general express or cold chain logistics.
4.When will the results/reports be available for the structural analysis project ?
A: For structural biology business, our technicians will assess the specificity of the protein. Whether the protein has been expressed by our company/reported in the literature/de novo protein or not, the project varies and the delivery time varies from 1 to 6 months.
The exact project delivery time will be answered after communication between customer and our technicians.
Technology
Structural Biology
1.What are the reasons for protein non-expression and low expression ?
A: It may be related to the stability of the protein, as proteins with low stability are prone to degradation, resulting in a low amount of protein obtained; it may also be related to the choice of expression system, as the prokaryotic expression system may result in some proteins not folding correctly, thus resulting in a low amount of protein obtained.
2. What should I do if I get inclusion body protein expression?
A: The appearance of inclusion bodies may generally be due to too fast protein expression or incorrect protein folding. Attempts can be made to optimize protein expression conditions, such as lowering the induction temperature, inducer concentration, and shortening the induction time to slow down protein expression. Or try to purify the inclusion body protein to turn it into an active protein.
3.What is the reason for protein non-crystallization ?
A: There are many reasons for the occurrence of protein non-crystallization. The protein itself may be not pure enough, homogeneous, unstable, with low protein concentration, or doesn’t reach saturation concentration. It may also be affected by external conditions, such as unsuitable temperature (protein crystallization can generally be performed at room temperature, but some proteins need to be crystallized at low temperature)
4.If I provide protein by myself, how much protein do I need to provide for structural analysis ?
A: Usually 10 mg of protein is required for screening.
5.How should I choose among these three structural analysis techniques (MicroED, cryo-electron microscopy, X-ray diffraction) ?
A: We will provide you with the best solution depending on the specifics of your sample and the needs of your project. 
In general, if your sample can be crystallized and the crystallization process is smooth, we recommend using conventional X-ray diffraction for structural analysis; if your sample can only form micro and nano crystals/powder crystals, we recommend using the MicroED technique to reduce the effort, time and cost consumed in the crystallization process and to obtain fast results; if your samples cannot be crystallized, or if crystallization cannot be applied to your project requirements, we recommend that you use cryo-electron microscopy for structural analysis.

Drug Solid State
1.What are the reasons for multiple peaks or small peak differences in XRPD profiles?
A: The XRPD multi-peak problem is a relatively frequent problem in crystallographic studies, where multiple peaks and small peak differences are often found in different batches of drugs. Usually, one or two small peak differences may occur when the crystal form of a sample contains a small amount of impurity crystals. When this occurs, the crystal structure of the compound can be obtained by single crystal culture and then its XRPD pattern can be simulated to confirm the crystalline purity of the sample. Another reason may be caused by crystal habit, where the same crystal form may exhibit different crystal morphologies. The relative intensities of XRPD diffraction peaks can vary explicitly from one crystal morphology to another due to the meritocratic orientation.
2.What is the cause of compound transcrystallization?
A: The transformation of a compound from one crystalline state to another is called transcrystallization. It is related to the physicochemical and mechanical properties of the solid form of the drug. These properties affect the solubility, solubility, stability, etc., of the drug, and transcrystallization can lead to changes in the properties of the drug, so the study of the phenomenon of transcrystallization of the solid state of the drug is also very important in the process of drug development. From drug raw materials to finished solid formulations, the intermediate process through multi-step processing (refining, crushing, granulation, drying, tablet pressing, etc.), these processes may make the drug crystal form change.
3.If I provide compounds by myself, how much do I need to provide for a crystal screening/salt screening/single crystal culture?
A: At least about 5g/5g/1g of compound should be provided for screening in crystal form screening/salt screening/single crystal culture.
4.How to choose crystal form screening or salt screening of compounds?
A: We will provide you with the best solution depending on the specifics of your sample and the needs of your project.
Usually, if your sample compounds have functional groups that can form salts, you can perform both crystal form screening and salt screening on the sample, and we will select the dominant crystal/salt form based on the preliminary screening and your project requirements.