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Organic molecules
In the past, the crystal structures of most organic molecules were determined by single crystal X diffraction, but this method requires the production of large and ordered crystals. If suitable size crystals cannot be produced, powder diffraction can be used to determine the structure. But it is more challenging than single crystal diffraction. MicroED can collect data from submicron sized crystals, making it easier to resolve the structure of organic molecules at the submicron level.
Technical Features – MicroED
MicroED is a structure analysis technique which is developed based on cryo-transmission electron microscopy. The electron diffraction data is collected and analyzed by diffracting electrons from tiny crystals. Extremely small crystal sizes, like micro- and nano-sized crystals, can produce diffraction signals with sufficiently high signal-to-noise ratios. High resolution diffraction data can be provided quickly and efficiently, and it significantly reduces the requirements for sample shape, purity and size.
Publication Share
The group of Frances H. Arnold at California Institute of Technology used MicroED technology to determine the structure of the ApePgb variant, which promotes the selective conversion of diazirines to carbene precursors. And they demonstrates the application of these highly stable carbene precursors in biocatalytic cyclopropanation.
Reference: Biocatalytic Carbene Transfer Using Diazirines
Service Flow
1.Requirement Confirmation
Our technical support and commercial colleagues will keep a smooth communication with you throughout the process. After establishing contact with you, we will understand your sample information and requirements in detail, and provide you with the best customized solution.
2.Sample Evaluation
You need to fill out the Sample Information Form and provide the information required by the technical team for evaluation. For example, providing powder diffraction data and SEM data, the technical team will then provide you with the sample evaluation results and preliminary feasibility program.
3. Sample Delivery
You can choose the appropriate shipping method according to the characteristics of samples.
4.Data Collection and Processing
We will prepare and process the samples according to the characteristics of the samples and the purpose of the experiment. The sample is placed under the electron microscope to find the appropriate sample particles, and series of clear and high quality electron diffraction point images will be collected.
5. Structure Analysis
We will use different algorithms, such as Patterson method, direct method, charge flip method, maximum entropy method, genetic algorithm, simulated annealing method, etc., to resolve the distribution of atoms in the crystal and obtain the initial structure of the crystal. We will further refine the initial structure of the crystal to obtain a chemically reasonable and experimentally observed structure, and finally deliver the results.