Structure Analysis

service

Structural Biology

  Tel:18962587269
  Email:contact@readcrystal.com
  Address:Changshu High-tech Industrial Development district, Suzhou, Jiangsu Province 1001, Building 6, No.88 Xian Shi Road

      Structure determines function. Proteins need to be folded into a specific spatial structure in order to have the appropriate physiological activity and biological function. Structure analysis is important for understanding the function of proteins, the interactions between biological macromolecules and for research into drug discovery.

      Structural biology is the core business of ReadCrystal. With our professional team and rich experience in structural analysis, we provide customized structural analysis solutions for your research and drug discovery research with a good service attitude.

     
      At ReadCrystal, we have outstanding experts in the field of X-ray crystallography to help you solve difficult problems in crystallography. In addition, we offer you the latest and most mature MicroED technology in the field of structure analysis for samples that are difficult to crystallize. ReadCrystal has developed the leading MicroED structure analysis platform and is working with experts from the founding team of MicroED at Stockholm University and Peking University to solve your crystallography problems.

X-ray Diffraction
        
X-ray diffraction is the main method for determining the structure of crystals, and the structural information of the crystal can be calculated from the captured diffraction direction and intensity signal data.

Technical features
•    Good homogeneity
•    Suitable for crystallizable samples
•    required crystal sizes down to 10μm
•    suitable for target structure resolution, protein and small molecule co-crystallization, antigen epitope identification

Testing process
  1. Customer can specify a protein so that we will express and purify the protein. Also, customer can provide proteins by themselves.
  2. Protein crystallization and crystallization protocol optimization
  3. Screening the crystallization to select single crystals of suitable size
  4. X-ray synchrotron radiation
  5. X-ray diffraction data analysis (including de-novo structure analysis and molecular replacement structure analysis)

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MicroED
MicroED is a cutting-edge technique based on cryo-electron microscopy. Traditional X-ray diffraction in structural biology requires high crystal size (typically >10 μm), while the cultivation of large crystals is usually difficult, time-consuming and uncertain. Many molecules can only form nanoscale crystals. MicroED technology can directly use nanoscale crystals to collect data and identify structures, thus greatly shortening the process of the most time-consuming and laborious wet experiments in structural research, saving experimental costs and optimizing experimental results.

Technical features
•    Protein crystals only need to be micro-nanometer in size, unlimited molecular weight
•    Suitable for processing membrane protein microcrystals
•    Very short experimental time, only 1 to 3 days for results
Testing process
  1. Customer can specify a protein so that we will express and purify the protein. Also, customer can provide proteins by themselves.
  2. Protein crystallization and crystallization protocol optimization
  3. Cryoprocessing, cryo-focused ion beam milling
  4. Diffraction data analysis
  5. MicroED data analysis (including de-novo protein molecular modeling and molecular replacement phase analysis)
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