Structural Biology
Molecular interactions include nucleic acid-nucleic acid interactions, nucleic acid-protein interactions and protein-protein interactions. Molecular interaction experiments enable visual interpretation of biological processes and molecular recognition processes, and elucidate the mechanisms of action between molecules, validate the ability of drug candidates to bind to target molecules, drive the discovery of drug leads and optimize the safety, efficacy, dosage form and manufacturing process of drugs.
ReadCrystal establishes a team of molecular interactions with extensive experience, providing a full range of service from protocol design to technical implementation for nucleic acid-nucleic acid, nucleic acid-protein and protein-protein interactions.
At a set stable reaction temperature, when a macromolecule/ligand conjugate is generated, the release or absorption of energy results in a change in cuvette temperature which is precisely detected and recorded. Typically, a complete ITC experiment involves determining reactant concentrations, titrating, collecting data, calibrating the raw data, deriving thermodynamic parameters from the calibrated data and analyzing the model.
• Protein-protein interactions
This technique is used for high throughput drug and target screening by measuring the thermal stability of proteins. DSF uses special fluorescent dyes as indicators, which bind and enhance fluorescence when the protein folding structure changes at elevated temperatures.
Molecular Interaction
• dentification of biosimilar candidates
• High throughput screening for enzyme stability
• Protein quality control
• Ligand binding assays
